The two actresses both picked up prizes for their "hotness" at the fifth annual "tribute to all things male", which took place at the Sony Pictures Studios in Los Angeles.
Aniston, who was wearing a black mini-dress, took home the previously-confirmed trophy for 'Decade of Hotness', and told the audience that she'd keep doing yoga for years to come.
Kunis was recognised as 'The Holy Grail Of Hotness', while Transformers: Dark of the Moon star Rosie Huntington-Whiteley was named 'Our New Girlfriend'.
Mark Wahlberg was named 'Guy of the Year' and his Oscar-nominated film The Fighter scooped 'Guy Movie Of The Year', while Jim Carrey was recognised as the 'Funniest M.F.' in the business.
Robert De Niro was on hand to induct Fast Times at Ridgemont High into the 'Guy Movie Hall of Fame'. He presented the honour to Sean Penn, Judge Reinhold, Forest Whitaker, Robert Romanus, Brian Backer and Amy Heckerling.
De Niro later presented the 'Brass Balls' gong to Keith Richards, who also took home the 'Outstanding Literary Achievement' prize for his memoir Life.
Other highlights from the star-studded ceremony included Harrison Ford and Jon Favreau accepting the prize for 'Most Manticipated Movie' for the upcoming Cowboys & Aliens from Jeremy Renner.
Scarlett Johansson, Cameron Diaz, Justin Timberlake, Eva Mendes, Samuel L. Jackson and Chris Evans were among the famous faces who also attended the awards show.
The Spike TV Guys Choice Awards air Friday, June 10 at 9pm ET/PT on Spike TV.
Guy of the Year
Mark Wahlberg
Brass Balls
Keith Richards
Decade of Hotness
Jennifer Aniston
Funniest M.F.
Jim Carrey
Guy Movie Hall of Fame
Fast Times at Ridgemont High
Most Manticipated Movie
Cowboys & Aliens
Guy Movie of the Year
The Fighter
Biggest Ass Kicker
Dwayne Johnson
Most Dangerous Man
Jon Jones
Holy Grail of Hot
Mila Kunis
Unstoppable Jock
Aaron Rodgers
Our New Girlfriend
Rosie Huntington-Whiteley
Top Fantasy Leaguer
Chris Paul
Comedy MVP
Danny McBride
Hot and Funny
Sofia Vergara
Outstanding Literary Achievement
Keith Richards, Life
Rookie Of The Year
Blake Griffin